Open AccessEmploying ProteoWizard to Convert Raw Mass Spectrometry Data
Author(s) -
Holman Jerry D.,
Tabb David L.,
Mallick Parag
Publication year - 2014
Publication title -
current protocols in bioinformatics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.535
H-Index - 57
eISSN - 1934-340X
pISSN - 1934-3396
DOI - 10.1002/0471250953.bi1324s46
Subject(s) - vendor , raw data , protocol (science) , computer science , software , coding (social sciences) , database , data format , mass spectrometry , software engineering , world wide web , operating system , programming language , computer hardware , chemistry , chromatography , medicine , statistics , alternative medicine , mathematics , pathology , marketing , business
After raw data have been captured by mass spectrometers in biological LC‐MS/MS experiments, they must be converted from vendor‐specific binary files to open‐format files for manipulation by most software. This protocol details the use of ProteoWizard software for this conversion, taking format features, coding options, and vendor particularities into account. This protocol will aid researchers in preparing their data for analysis by database search engines and other bioinformatics tools. Curr. Protoc. Bioinform . 46:13.24.1‐13.24.9. © 2014 by John Wiley & Sons, Inc.