Open AccessIsolation and Culturing of Glioma Cancer Stem Cells
Author(s) -
Kim SangSoo,
Pirollo Kathleen F.,
Chang Esther H.
Publication year - 2015
Publication title -
current protocols in cell biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.149
H-Index - 38
eISSN - 1934-2616
pISSN - 1934-2500
DOI - 10.1002/0471143030.cb2310s67
Subject(s) - cancer stem cell , glioma , glioblastoma , cancer research , metastasis , cancer , isolation (microbiology) , drug resistance , brain cancer , cancer cell , cancer metastasis , biology , medicine , bioinformatics , microbiology and biotechnology
In many human cancers including malignant glioblastoma multiforme (GBM), cancer stem cells (CSCs) are thought to be responsible for tumor initiation, metastasis and resistance to conventional anti‐cancer therapies. Therefore, a CSC‐targeted drug delivery strategy to eliminate CSCs is a desirable approach for developing a more effective therapeutic. Moreover, isolated CSCs will provide an invaluable tool for studying the underlying cellular mechanisms of tumor development and provide insight into therapeutic options for successful eradication of CSCs. This unit describes a method for the isolation and culture of CSCs from human GBM tumor tissue. © 2015 by John Wiley & Sons, Inc.