Open AccessExpanding Mouse Ventricular Cardiomyocytes Through GSK‐3 Inhibition
Author(s) -
Buikema Jan W.,
Zwetsloot PeterPaul M.,
Doevendans Pieter A.,
Sluijter Joost P.G.,
Domian Ibrahim J.
Publication year - 2013
Publication title -
current protocols in cell biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.149
H-Index - 38
eISSN - 1934-2616
pISSN - 1934-2500
DOI - 10.1002/0471143030.cb2309s61
Subject(s) - wnt signaling pathway , regenerative medicine , microbiology and biotechnology , population , biology , in vivo , computational biology , signal transduction , stem cell , medicine , environmental health
Controlled proliferation of cardiomyocytes remains a major limitation in cell biology and one of the main underlying hurdles for true modern regenerative medicine. Here, a technique is described for robust expansion of early fetal‐derived mouse ventricular cardiomyocytes on a platform usable for high‐throughput molecular screening, tissue engineering and, potentially, in vivo translational experiments. This method provides a small‐molecule approach to control proliferation or differentiation of early beating cardiomyocytes through modulation of the Wnt/β‐catenin signaling pathway. Moreover, isolation and expansion of fetal cardiomyocytes takes less than 3 weeks, yields a relatively pure (∼70%) functional myogenic population, and is highly reproducible. Curr. Protoc. Cell Biol . 61:23.9.1‐23.9.10. © 2013 by John Wiley & Sons, Inc.