Open AccessDesign and Use of Fluorescent Fusion Proteins in Cell Biology
Author(s) -
Snapp Erik
Publication year - 2005
Publication title -
current protocols in cell biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.149
H-Index - 38
eISSN - 1934-2616
pISSN - 1934-2500
DOI - 10.1002/0471143030.cb2104s27
Subject(s) - fluorescence , fluorescent protein , green fluorescent protein , microbiology and biotechnology , fusion protein , fusion , biology , computational biology , biophysics , biochemistry , physics , recombinant dna , optics , gene , linguistics , philosophy
This unit describes strategies for designing functional fluorescent fusion protein constructs. Such constructs can be exploited as probes of cellular environments, protein dynamics, protein life histories, protein binding partners, and markers in living cells. The properties and uses of many currently available fluorescent proteins are discussed. In addition, alternative approaches and troubleshooting guidelines are provided.