Open AccessTools for Controlling Protein Interactions Using Light
Author(s) -
Tucker Chandra L.,
Vrana Justin D.,
Kennedy Matthew J.
Publication year - 2014
Publication title -
current protocols in cell biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.149
H-Index - 38
eISSN - 1934-2616
pISSN - 1934-2500
DOI - 10.1002/0471143030.cb1716s64
Subject(s) - computer science , computational biology , biology
Genetically encoded actuators that allow control of protein‐protein interactions using light, termed ‘optical dimerizers’, are emerging as new tools for experimental biology. In recent years, numerous new and versatile dimerizer systems have been developed. Here we discuss the design of optical dimerizer experiments, including choice of a dimerizer system, photoexcitation sources, and the coordinate use of imaging reporters. We provide detailed protocols for experiments using two dimerization systems we previously developed, CRY2/CIB and UVR8/UVR8, for use in controlling transcription, protein localization, and protein secretion using light. Additionally, we provide instructions and software for constructing a pulse‐controlled LED device for use in experiments requiring extended light treatments. Curr. Protoc. Cell Biol . 64:17.16.1‐17.16.20. © 2014 by John Wiley & Sons, Inc.