Open AccessChromatin Immunoprecipitation of Adult Murine Cardiomyocytes
Author(s) -
Bolli P.,
Vardabasso C.,
Bernstein E.,
Chaudhry H.W.
Publication year - 2013
Publication title -
current protocols in cell biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.149
H-Index - 38
eISSN - 1934-2616
pISSN - 1934-2500
DOI - 10.1002/0471143030.cb1714s58
Subject(s) - chromatin immunoprecipitation , epigenetics , immunoprecipitation , chromatin , microbiology and biotechnology , biology , computational biology , epigenesis , dna , dna methylation , gene , genetics , gene expression , promoter
This unit describes a streamlined two‐step protocol for the isolation of adult murine cardiomyocytes with subsequent Chromatin ImmunoPrecipitation (ChIP). Isolation and culturing of cardiomyocytes is a delicate process and the protocol presented here optimizes the combination of cardiomyocyte isolation with ChIP. ChIP is an invaluable method for analyzing molecular interactions occurring between a specific protein (or its post‐translationally modified form) and a region of genomic DNA. ChIP has become a widely used technique in the last decade since several groundbreaking studies have focused attention on epigenetics and have identified many epigenetic regulatory mechanisms. However, epigenetics within cardiovascular biology is a new area of focus for many investigators, and we have optimized a method for performing ChIP in adult murine cardiomyocytes, as we feel this will be an important aid to both the cardiovascular field and for the development of cell‐ and tissue‐specific ChIP. Curr. Protoc. Cell Biol . 58:17.14.1‐17.14.16. © 2013 by John Wiley & Sons, Inc.