Immunofluorescence Staining | Zendy

Donaldson Julie G. | Zendy

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Immunofluorescence Staining

Author(s) -

Donaldson Julie G.

Publication year - 1998

Publication title -

current protocols in cell biology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.149

H-Index - 38

eISSN - 1934-2616

pISSN - 1934-2500

DOI - 10.1002/0471143030.cb0403s00

Subject(s) - immunofluorescence , fluorophore , fluorescence , molecule , antibody , staining , fluorescence microscope , chemistry , direct fluorescent antibody , primary and secondary antibodies , microbiology and biotechnology , biophysics , biology , optics , immunology , physics , genetics , organic chemistry

This unit provides a protocol for indirect immunofluorescence, which is a method that provides information about the locations of specific molecules and the structure of the cell. Antibody molecules for a specific target molecule are exposed to the cell or tissue being investigated. The binding of these molecules is detected by incubating the sample with a secondary antibody specific for immunoglobulin molecules and conjugated to fluorophore. This provides both a visible signal and amplification of the signal and the results are observed with a fluorescent microscope.

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