Open AccessIsolation of Aggresomes and Other Large Aggregates
Author(s) -
Meriin Anatoli B.,
Wang Yan,
Sherman Michael Y.
Publication year - 2010
Publication title -
current protocols in cell biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.149
H-Index - 38
eISSN - 1934-2616
pISSN - 1934-2500
DOI - 10.1002/0471143030.cb0338s48
Subject(s) - aggresome , microtubule , organelle , chemistry , microbiology and biotechnology , protein aggregation , biology , biophysics , biochemistry , ubiquitin , gene
Upon permanent stresses and in various diseases, small protein aggregates may accumulate in cells and cause toxicity. A recently discovered protective system transports these aggregates to the centrosome location via microtubules, to form a large agglomerate of aggregates called the aggresome. Here, we describe a newly developed method for isolating aggresomes. This principle can also be used for purification of other large structures and even organelles. Curr. Protoc. Cell Biol . 48:3.38.1‐3.38.9. © 2010 by John Wiley & Sons, Inc.