Open AccessAssessing Mitochondrial Redox Status by Flow Cytometric Methods: Vascular Response to Fluid Shear Stress
Author(s) -
Li Rongsong,
Jen Nelson,
Yu Fei,
Hsiai Tzung K.
Publication year - 2011
Publication title -
current protocols in cytometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.718
H-Index - 26
eISSN - 1934-9300
pISSN - 1934-9297
DOI - 10.1002/0471142956.cy0937s58
Subject(s) - oxidative stress , mitochondrion , microbiology and biotechnology , superoxide , intracellular , reactive oxygen species , apoptosis , redox , biology , oxidative phosphorylation , chemistry , biochemistry , enzyme , organic chemistry
Mitochondria are an important source of superoxide production contributing to physiological and pathological responses, including vascular oxidative stress that is relevant to cardiovascular diseases. Vascular oxidative stress is intimately linked with pro‐inflammatory states and atherosclerosis. Oxidized low‐density lipoprotein (OxLDL) modulates intracellular redox status and induces apoptosis in endothelial cells. Hemodynamic, specifically, fluid shear stress imparts both biomechanical and metabolic effects on vasculature. Mitochondria are an important source of superoxide production contributing to vascular oxidative stress with relevance to cardiovascular diseases. We hereby present biophysical and biochemical approaches, including fluorescence‐activated cell sorting, to assess the dynamics of vascular redox status. Curr. Protoc. Cytom . 58:9.37.1‐9.37.14. © 2011 by John Wiley & Sons, Inc.