Open AccessMouse Genome Editing Using the CRISPR/Cas System
Author(s) -
Harms Donald W.,
Quadros Rolen M.,
Seruggia Davide,
Ohtsuka Masato,
Takahashi Gou,
Montoliu Lluis,
Gurumurthy Channabasavaiah B.
Publication year - 2014
Publication title -
current protocols in human genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.282
H-Index - 30
eISSN - 1934-8258
pISSN - 1934-8266
DOI - 10.1002/0471142905.hg1507s83
Subject(s) - crispr , genome editing , computational biology , biology , model organism , genome , genetics , gene
The availability of techniques to create desired genetic mutations has enabled the laboratory mouse as an extensively used model organism in biomedical research including human genetics. A new addition to this existing technical repertoire is the CRISPR/Cas system. Specifically, this system allows editing of the mouse genome much more quickly than the previously used techniques, and, more importantly, multiple mutations can be created in a single experiment. Here we provide protocols for preparation of CRISPR/Cas reagents and microinjection into one‐cell mouse embryos to create knockout or knock‐in mouse models. Curr. Protoc. Hum. Genet . 83:15.7.1‐15.7.27. © 2014 by John Wiley & Sons, Inc.