Open AccessStranded Whole Transcriptome RNA‐Seq for All RNA Types
Author(s) -
Miller David F.B.,
Yan Pearlly X.,
Fang Fang,
Buechlein Aaron,
Ford James B.,
Tang Haixu,
Huang Tim H.,
Burow Matthew E.,
Liu Yunlong,
Rusch Douglas B.,
Nephew Kenneth P.
Publication year - 2015
Publication title -
current protocols in human genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.282
H-Index - 30
eISSN - 1934-8258
pISSN - 1934-8266
DOI - 10.1002/0471142905.hg1114s84
Subject(s) - rna , non coding rna , rna editing , rna silencing , transcriptome , biology , transfer rna , piwi interacting rna , small nucleolar rna , computational biology , microrna , rna seq , small nuclear rna , genetics , gene expression , rna interference , gene
Abstract Stranded whole transcriptome RNA‐Seq described in this unit captures quantitative expression data for all types of RNA including, but not limited to, miRNA (microRNA), piRNA (Piwi‐interacting RNA), snoRNA (small nucleolar RNA), lincRNA (large non‐coding intergenic RNA), SRP RNA (signal recognition particle RNA), tRNA (transfer RNA), mtRNA (mitochondrial RNA), and mRNA (messenger RNA). The size and nature of these types of RNA are irrelevant to the approach described here. Barcoded libraries for multiplexing on the Illumina platform are generated with this approach but it can be applied to other platforms with a few modifications. © 2015 by John Wiley & Sons, Inc.