Open AccessQuantitative Analysis of Copy Number Variants Based on Real‐Time LightCycler PCR
Author(s) -
Ma Lijiang,
Chung Wendy K.
Publication year - 2014
Publication title -
current protocols in human genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.282
H-Index - 30
eISSN - 1934-8258
pISSN - 1934-8266
DOI - 10.1002/0471142905.hg0721s80
Subject(s) - real time polymerase chain reaction , copy number variation , hum , gene , biology , genetics , gene dosage , dna , microbiology and biotechnology , copy number analysis , gene duplication , computational biology , gene expression , genome , art , performance art , art history
Quantitative real‐time PCR is PCR visualized in real time by the use of fluorescent or intercalating dyes, which are employed to measure gene expression or gene quantification including contiguous gene deletions or duplications. A simple method is described here to quantify DNA copy number from human samples. Curr. Protoc. Hum. Genet . 80:7.21.1‐7.21.8. © 2014 by John Wiley & Sons, Inc.