Open AccessTrypan Blue Exclusion Test of Cell Viability
Author(s) -
Strober Warren
Publication year - 2015
Publication title -
current protocols in immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.618
H-Index - 43
eISSN - 1934-368X
pISSN - 1934-3671
DOI - 10.1002/0471142735.ima03bs111
Subject(s) - trypan blue , cytoplasm , viability assay , cell , microbiology and biotechnology , cell counting , biology , chemistry , biochemistry , cell cycle
The protocol described in this appendix allows for light microscopic quantitation of cell viability. Cells are suspended in PBS containing trypan blue and then examined to determine the percentage of cells that have clear cytoplasm (viable cells) versus cells that have blue cytoplasm (nonviable cells). © 2015 by John Wiley & Sons, Inc.