B16 as a Mouse Model for Human Melanoma | Zendy

Overwijk Willem W. | Zendy; Restifo Nicholas P. | Zendy

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B16 as a Mouse Model for Human Melanoma

Author(s) -

Overwijk Willem W.,

Restifo Nicholas P.

Publication year - 2000

Publication title -

current protocols in immunology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.618

H-Index - 43

eISSN - 1934-368X

pISSN - 1934-3671

DOI - 10.1002/0471142735.im2001s39

Subject(s) - ctl* , melanoma , adoptive cell transfer , cytotoxic t cell , vitiligo , immunology , medicine , in vivo , immunotherapy , recombinant dna , antibody , vaccination , cancer research , biology , in vitro , immune system , t cell , cd8 , biochemistry , microbiology and biotechnology , gene

This unit details protocols for in vivo models of subcutaneous growth and pulmonary metastases of B16 melanoma. Therapeutic approaches include the use of B16.GM‐CSF and rVVmTRP‐1 to induce autoimmune vitiligo and tumor protection. The induction and use of gp100‐specific therapeutic cytotoxic T lymphocytes (CTL) are discussed. Methods are also included for CTL induction, isolation and testing, CTL maintenance, and adoptive transfer. Support protocols detail the testing of mouse sera for presence of MDA‐specific antibodies by immunoblotting and ELISA, respectively. Additional sections, including growing B16 melanoma, enumerating pulmonary metastases, and use of recombinant viruses for vaccination, are discussed together with safety concerns.

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