Open AccessSampling of Intestinal Microbiota and Targeted Amplification of Bacterial 16S rRNA Genes for Microbial Ecologic Analysis
Author(s) -
Tong Maomeng,
Jacobs Jonathan P.,
McHardy Ian H.,
Braun Jonathan
Publication year - 2014
Publication title -
current protocols in immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.618
H-Index - 43
eISSN - 1934-368X
pISSN - 1934-3671
DOI - 10.1002/0471142735.im0741s107
Subject(s) - biology , dysbiosis , 16s ribosomal rna , microbial ecology , gut flora , operational taxonomic unit , ribosomal rna , microbiology and biotechnology , phylogenetic tree , metagenomics , gene , computational biology , bacteria , microbiome , genetics , immunology
Dysbiosis of host‐associated commensal microbiota is emerging as an important factor in risk and phenotype of immunologic, metabolic, and behavioral diseases. Accurate analysis of microbial composition and functional state in humans or mice requires appropriate collection and pre‐processing of biospecimens. Methods to sample luminal and mucosal microbiota from human or mouse intestines and to profile microbial phylogenetic composition using 16S rRNA sequencing are presented here. Data generated using the methods in this unit can be used for downstream quantitative analysis of microbial ecology. © 2014 by John Wiley & Sons, Inc.