Open AccessDifferentiation and Characterization of Tr1 Cells
Author(s) -
Chihara Norio,
Madi Asaf,
Karwacz Katarzyna,
Awasthi Amit,
Kuchroo Vijay K.
Publication year - 2016
Publication title -
current protocols in immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.618
H-Index - 43
eISSN - 1934-368X
pISSN - 1934-3671
DOI - 10.1002/0471142735.im0327s113
Subject(s) - characterization (materials science) , chemistry , microbiology and biotechnology , materials science , nanotechnology , biology
Regulatory T cell–mediated suppression serves as a pivotal mechanism of negative regulation of immune‐mediated inflammation. Type 1 regulatory T cells (Tr1 cells) are an important subset of CD4+ T cells that prevent excessive inflammatory responses and maintain immune tolerance. The anti‐inflammatory role of Tr1 cells is mediated in part by their production of interleukin 10 (IL‐10), which dampens the function of both antigen‐presenting cells and antigen‐specific effector T cells. Additionally, Tr1 cells can kill effector and myeloid cells through the perforin‐granzyme B pathway. Adoptive transfer of in vitro differentiated Tr1 cells can be used to suppress autoimmune tissue inflammation in vivo. This unit describes the in vitro stimulation of naïve murine CD4+ T cells using IL‐27 to generate IL‐10–producing Tr1 cells. © 2016 by John Wiley & Sons, Inc.