Open AccessVaccination of Mice with Baculovirus‐Infected Insect Cells Expressing Antigenic Proteins
Author(s) -
Jordan Kimberly R.,
Crawford Fran,
Kappler John W.,
Slansky Jill E.
Publication year - 2009
Publication title -
current protocols in immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.618
H-Index - 43
eISSN - 1934-368X
pISSN - 1934-3671
DOI - 10.1002/0471142735.im0215s85
Subject(s) - antigen , immune system , adjuvant , biology , virology , cytotoxic t cell , vaccination , immunization , antibody , t cell , immunology , recombinant dna , gene , in vitro , genetics
Methods to induce antigen‐specific immune responses in mice using insect cells infected with recombinant baculoviruses are described in this unit. Although this vaccine strategy has been used to generate both antibody and T cell responses, it has been more thoroughly characterized for the peptide‐specific cytotoxic T cell responses. Nonspecific responses to the vaccine vehicle are controlled for by vaccinating with insect cells infected with baculoviruses encoding irrelevant antigens or no antigen. The baculovirus‐infected insect cells alone are an effective immune adjuvant to elicit antigen‐specific T cells. Overall, immune responses generated using this approach are similar to those generated by more conventional vaccine strategies. Curr. Protoc. Immunol . 85:2.15.1‐2.15.23. © 2009 by John Wiley & Sons, Inc.