Open AccessOne‐Flask Synthesis of Cyclic Diguanosine Monophosphate (c‐di‐GMP)
Author(s) -
Gaffney Barbara L.,
Jones Roger A.
Publication year - 2012
Publication title -
current protocols in nucleic acid chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.306
H-Index - 17
eISSN - 1934-9289
pISSN - 1934-9270
DOI - 10.1002/0471142700.nc1408s48
Subject(s) - biofilm , nucleic acid , virulence , chemistry , cyclic gmp , bacteria , biochemistry , microbiology and biotechnology , biology , enzyme , genetics , gene
The bacterial signaling molecule, cyclic diguanosine monophosphate (c‐di‐GMP), plays a key role in controlling biofilm formation and pathogenic virulence, among many other functions. It has widespread consequences for human health, and current research is actively exploring its molecular mechanisms. The convenient one‐flask, gram‐scale synthesis of c‐di‐GMP described here will facilitate these efforts. Curr. Protoc. Nucleic Acid Chem . 48:14.8.1‐14.8.7. © 2012 by John Wiley & Sons, Inc.