Open AccessNMR Analysis of Base‐Pair Opening Kinetics in DNA
Author(s) -
Szulik Marta W.,
Voehler Markus,
Stone Michael P.
Publication year - 2014
Publication title -
current protocols in nucleic acid chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.306
H-Index - 17
eISSN - 1934-9289
pISSN - 1934-9270
DOI - 10.1002/0471142700.nc0720s59
Subject(s) - base pair , kinetics , dna , base (topology) , nucleic acid , nuclear magnetic resonance spectroscopy , closing (real estate) , double stranded , chemistry , biophysics , nuclear magnetic resonance , stereochemistry , physics , biology , biochemistry , mathematics , mathematical analysis , quantum mechanics , political science , law
Base pairing in nucleic acids plays a crucial role in their structure and function. Differences in the base‐pair opening and closing kinetics of individual double‐stranded DNA sequences or between chemically modified base pairs provide insight into the recognition of these base pairs by DNA processing enzymes. This unit describes how to quantify the kinetics for localized base pairs by observing changes in the imino proton signals by nuclear magnetic resonance spectroscopy. The determination of all relevant parameters using state‐of‐the art techniques and NMR instrumentation, including cryoprobes, is discussed. © 2014 by John Wiley & Sons, Inc.