Open AccessSynthesis of the Tellurium‐Derivatized Phosphoramidites and Their Incorporation into DNA Oligonucleotides
Author(s) -
Jiang Sibo,
Sheng Jia,
Huang Zhen
Publication year - 2011
Publication title -
current protocols in nucleic acid chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.306
H-Index - 17
eISSN - 1934-9289
pISSN - 1934-9270
DOI - 10.1002/0471142700.nc0125s47
Subject(s) - oligonucleotide , tellurium , dna , chemistry , combinatorial chemistry , computational biology , biology , biochemistry , organic chemistry
In this unit, an efficient method for the synthesis of 2′‐tellerium‐modified phosphoramidite and its incorporation into oligonucleotide are presented. We choose 5′‐O‐DMTr‐2,2′‐anhydro‐uridine and ‐thymidine nucleosides ( S.1, S.2 ) as starting materials due to their easy preparation. The 5′‐O‐DMTr‐2,2′‐anhydro‐uridine and ‐thymidine can be converted to the corresponding 2′‐tellerium‐derivatized nucleosides by treating with the telluride nucleophiles. Subsequently, the 2′‐Te‐nucleosides can be transformed into 3′‐phosphoramidites, which are the building blocks for DNA/RNA synthesis. The DNA synthesis, purification, and applications of oligonucleotides containing 2′‐Te‐U or 2′‐Te‐T are described in the protocol. Curr. Protoc. Nucleic Acid Chem . 47:1.25.1‐1.25.16. © 2011 by John Wiley & Sons, Inc.