Open AccessThe Use of Herpes Simplex Virus in Ex Vivo Slice Culture
Author(s) -
Friedman Allyson K.,
Han MingHu
Publication year - 2015
Publication title -
current protocols in neuroscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.307
H-Index - 40
eISSN - 1934-8576
pISSN - 1934-8584
DOI - 10.1002/0471142301.ns0436s72
Subject(s) - herpes simplex virus , ex vivo , slice preparation , biology , gene expression , neuroscience , in vivo , virus , microbiology and biotechnology , gene , virology , electrophysiology , genetics
Herpes simplex virus (HSV) can be used for a wide range of genetic manipulations in ex vivo slices of central nervous system tissue from both young and adult rodents. The fast expression of the HSV viral‐mediated gene transfer, which can be engineered to produce cell‐type specificity, can be utilized in slice cultures for a variety of purposes over a 1‐ to 4‐day period with spatial and temporal specificity. This protocol exploits the rapid expression of HSV viral vectors by utilizing slice culture for electrophysiological recordings, avoiding the need to do intracranial viral injections. Brain slice cultures maintain many aspects of in vivo biology, including functional local synaptic circuitry with preserved brain architecture, while allowing good experimental access and precise control of the extracellular environment, making them ideal platforms for quick access to evaluate expression effects of HSV viral‐mediated gene transfer on the molecular and cellular properties of specific neurons. This protocol provides an easy way to study neuronal function following viral expression of a gene of interest. © 2015 by John Wiley & Sons, Inc.