Open AccessUtility and Applications of Orthotopic Models of Human Non‐Small Cell Lung Cancer (NSCLC) for the Evaluation of Novel and Emerging Cancer Therapeutics
Author(s) -
Justilien Verline,
Fields Alan P.
Publication year - 2013
Publication title -
current protocols in pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.893
H-Index - 26
eISSN - 1934-8290
pISSN - 1934-8282
DOI - 10.1002/0471141755.ph1427s62
Subject(s) - lung cancer , medicine , cancer , chemotherapy , radiation therapy , treatment of lung cancer , cancer research , oncology , in vivo , bioinformatics , biology , microbiology and biotechnology
Lung cancer is a leading cause of cancer deaths worldwide. Despite advances in chemotherapy, radiation therapy, and surgery, lung cancer continues to have a low 5‐year survival rate, highlighting a dire need for more effective means of prevention, diagnosis, prognosis, and treatment. Mouse models that recapitulate the clinical features of advanced human lung cancer are critical for testing novel therapeutic approaches. This unit describes a highly reproducible, easy‐to‐establish orthotopic murine model of lung cancer, provides methods for in vivo imaging and monitoring of tumor growth, and discusses the usefulness of this model for translational lung cancer research and the development of therapeutic strategies. Curr. Protoc. Pharmacol . 62:14.27.1‐14.27.17. © 2013 by John Wiley & Sons, Inc.