Open AccessCharacterization of GABA Receptors
Author(s) -
Enna S.J.,
McCarson Kenneth E.
Publication year - 2013
Publication title -
current protocols in pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.893
H-Index - 26
eISSN - 1934-8290
pISSN - 1934-8282
DOI - 10.1002/0471141755.ph0107s63
Subject(s) - homomeric , gabaa receptor , gabab receptor , receptor , chemistry , ligand gated ion channel , gabaa rho receptor , ion channel , binding site , ligand (biochemistry) , biophysics , biochemistry , pharmacology , biology , protein subunit , gene
Described in this unit are ligand‐binding assays for GABA A , GABA B , and the homomeric ρ GABA A (formerly GABA C ) receptor recognition sites in brain tissue. Although GABA binding sites are present in peripheral organs, most research is directed toward examining these receptors in the CNS. These assays may also be used to determine the affinity of an unlabeled compound for the GABA binding sites. Excluded from the unit are ligand‐binding assays for other components of the GABA A receptor complex, such as the benzodiazepine or ion‐channel binding sites. Curr. Protoc. Pharmacol . 63:1.7.1‐1.7.20. © 2013 by John Wiley & Sons, Inc.