Open AccessIn Situ Proteolysis for Crystallization of Membrane Bound Cytochrome P450 17A1 and 17A2 Proteins from Zebrafish
Author(s) -
Lei Li,
Egli Martin
Publication year - 2016
Publication title -
current protocols in protein science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.409
H-Index - 32
eISSN - 1934-3663
pISSN - 1934-3655
DOI - 10.1002/0471140864.ps2916s84
Subject(s) - proteolysis , hydroxylation , biochemistry , cytochrome p450 , proteases , membrane protein , integral membrane protein , biology , chemistry , enzyme , membrane
Fish and human cytochrome P450 (P450) 17A1 catalyze both steroid 17α‐hydroxylation and 17α,20‐lyase reactions. Fish P450 17A2 catalyzes only 17α‐hydroxylation. Both enzymes are microsomal‐type P450s, integral membrane proteins that bind to the membrane through their N‐terminal hydrophobic segment, the signal anchor sequence. The presence of this N‐terminal region renders expression of full‐length proteins challenging or impossible. For some proteins, variable truncation of the signal anchor sequence precludes expression or results in poor expression levels. To crystallize P450 17A1 and 17A2 in order to gain insight into their different activities, we used an alternative N‐terminal sequence to boost expression together with in situ proteolysis. Key features of our approach to identify crystallizable P450 fragments were the use of an N‐terminal leader sequence, a screen composed of 12 proteases to establish optimal cleavage, variations of protease concentration in combination with an SDS‐PAGE assay, and analysis of the resulting fragments using Edman sequencing. Described in this unit are protocols for vector preparation, expression, purification, and in situ proteolytic crystallization of two membrane‐bound P450 proteins. © 2016 by John Wiley & Sons, Inc.