Open AccessMethods for the Design and Analysis of Sedimentation Velocity and Sedimentation Equilibrium Experiments with Proteins
Author(s) -
Demeler Borries
Publication year - 2010
Publication title -
current protocols in protein science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.409
H-Index - 32
eISSN - 1934-3663
pISSN - 1934-3655
DOI - 10.1002/0471140864.ps0713s60
Subject(s) - analytical ultracentrifugation , ultracentrifuge , sedimentation equilibrium , sedimentation , sedimentation coefficient , chemistry , chromatography , biochemistry , biology , paleontology , sediment , enzyme
Analytical ultracentrifugation experiments play an integral role in the solution phase characterization of recombinant proteins and other biological macromolecules. This unit discusses the design of sedimentation velocity and sedimentation equilibrium experiments performed with a Beckman Optima XL‐A or XL‐I analytical ultracentrifuge. Optimal instrument settings and experimental design considerations are explained, and strategies for the analysis of experimental data with the UltraScan data analysis software package are presented. Special attention is paid to the strengths and weaknesses of the available detectors, and guidance is provided on how to extract maximum information from analytical ultracentrifugation experiments. Curr. Protoc. Protein Sci . 60:7.13.1‐7.13.24. © 2010 by John Wiley & Sons, Inc.