Open AccessOverview of Protein Expression by Mammalian Cells
Author(s) -
Gray David
Publication year - 1997
Publication title -
current protocols in protein science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.409
H-Index - 32
eISSN - 1934-3663
pISSN - 1934-3655
DOI - 10.1002/0471140864.ps0509s10
Subject(s) - transfection , protein expression , microbiology and biotechnology , recombinant dna , protein biosynthesis , intracellular , biology , cell culture , gene , biochemistry , genetics
This unit reviews the stages involved in protein production in mammalian cells using a stable‐expression approach. Choice of cell type is discussed, as is transfection of the host cells, methods for selection and amplification of transformants, and growth of cells at appropriate scale for protein production. Since post‐transcriptional modification and intracellular protein transportation are important features of recombinant‐protein production in mammalian cells, some description of these mechanisms is included.