Open AccessHigh‐Resolution Multi‐Photon Imaging of Morphological Structures of Caenorhabditis elegans
Author(s) -
Bixel Gabriele M.,
Fretham Stephanie J.B.,
Aschner Michael
Publication year - 2015
Publication title -
current protocols in toxicology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.449
H-Index - 23
eISSN - 1934-9262
pISSN - 1934-9254
DOI - 10.1002/0471140856.tx1119s64
Subject(s) - caenorhabditis elegans , second harmonic generation , excitation , photon , two photon excitation microscopy , optics , harmonic , physics , fluorescence , laser , biology , acoustics , biochemistry , quantum mechanics , gene
In this protocol, we combine two‐photon excitation fluorescence with nonlinear optical measurements to reconstruct the three‐dimensional architecture of the pharyngeal region and the muscular system of the anterior and mid‐body region of Caenorhabditis elegans ( C. elegans ). Femto‐second laser pulses excite second‐harmonic generation (SHG) and third‐harmonic generation (THG) signals, which show detailed structural information regarding the organization of myofibrils that are arranged around the central pharynx region. The combination of two‐photon excitation with SHG and THG imaging is a very powerful tool to study cell morphology, microarchitecture, and tissue arrangement in C. elegans . © 2015 by John Wiley & Sons, Inc.