Open AccessF 3 ‐Isoprostanes as a Measure of in vivo Oxidative Damage in Caenorhabditis elegans
Author(s) -
Nguyen Thuy T.,
Aschner Michael
Publication year - 2014
Publication title -
current protocols in toxicology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.449
H-Index - 23
eISSN - 1934-9262
pISSN - 1934-9254
DOI - 10.1002/0471140856.tx1117s62
Subject(s) - arachidonic acid , caenorhabditis elegans , eicosapentaenoic acid , polyunsaturated fatty acid , in vivo , oxidative stress , chemistry , lipid peroxidation , biochemistry , isoprostanes , oxidative phosphorylation , biology , fatty acid , gene , enzyme , microbiology and biotechnology
Oxidative stress has been implicated in the development of a wide variety of disease processes, including cardiovascular disease, cancer, and neurodegenerative diseases, as well as progressive and normal aging processes. Isoprostanes (IsoPs) are prostaglandin‐like compounds that are generated in vivo from lipid peroxidation of arachidonic acid (AA, C20:4, ω‐6) and other polyunsaturated fatty acids (PUFA). Since the discovery of IsoPs by Morrow and Roberts in 1990, quantification of IsoPs has been shown to be an excellent source of biomarkers of in vivo oxidative damage. Eicosapentaenoic acid (EPA, C20:5, ω‐3) is the most abundant PUFA in Caenorhabditis elegans and gives rise to F 3 ‐IsoPs upon nonenzymatic free‐radical‐catalyzed lipid peroxidation. The protocol presented is the current methodology that our laboratory uses to quantify F 3 ‐IsoPs in C. elegans using gas chromatography/mass spectrometry (GC/MS). The methods described herein have been optimized and validated to provide the best sensitivity and selectivity for quantification of F 3 ‐IsoPs from C. elegans lysates. © 2014 by John Wiley & Sons, Inc.