Measurement of Peroxiredoxin Activity | Zendy

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Measurement of Peroxiredoxin Activity

Author(s) -

Nelson Kimberly J.,

Parsonage Derek

Publication year - 2011

Publication title -

current protocols in toxicology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.449

H-Index - 23

eISSN - 1934-9262

pISSN - 1934-9254

DOI - 10.1002/0471140856.tx0710s49

Subject(s) - peroxiredoxin , peroxynitrite , hydrogen peroxide , horseradish peroxidase , thioredoxin , peroxidase , thioredoxin reductase , chemistry , biochemistry , peroxide , cysteine , enzyme , organic chemistry , superoxide

Peroxiredoxins are cysteine‐dependent peroxidases that react with hydrogen peroxide, larger hydroperoxide substrates, and peroxynitrite. Protocols are provided to measure Prx activity with peroxide by (1) a coupled reaction with NADPH, thioredoxin reductase, and thioredoxin, (2) the direct monitoring of thioredoxin oxidation, (3) competition with horseradish peroxidase, and (4) peroxide consumption using the FOX assay. Curr. Protoc. Toxicol . 49:7.10.1‐7.10.28. © 2011 by John Wiley & Sons, Inc.

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