Open AccessIn Vitro Growth Suppression by Adenoviral Transduction of p21 and p16 in Squamous Cell Carcinoma of the Head and Neck
Author(s) -
Steven Ross Mobley,
Ta-Jen Liu,
J. Michael Hudson,
Gary L. Clayman
Publication year - 1998
Publication title -
archives of otolaryngology - head and neck surgery
Language(s) - English
Resource type - Journals
eISSN - 1538-361X
pISSN - 0886-4470
DOI - 10.1001/archotol.124.1.88
Subject(s) - cell cycle , biology , cell growth , cancer research , cell culture , cell , viral vector , genetic enhancement , microbiology and biotechnology , transduction (biophysics) , gene , genetics , recombinant dna , biochemistry
To determine the duration of expression of the cell cycle regulators p21 and p16 and the effect of these 2 genes both alone and in combination on the growth of squamous cell carcinoma of the head and neck cell lines in vitro. EXPERIMENTAL METHODS AND DESIGN: Cells were transduced via an adenoviral vector with p21 (Ad5CMV-p21), p16 (Ad5CMV-p16), or both. Western blotting was performed to determine duration of expression of the protein products of the transduced p21 and p16 genes. In vitro growth assays and cell cycle analyses were performed on transduced cells.
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