English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Zendy Plus

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Zendy Tools

Zendy Open

A conserved  N -glycan-dependent endoplasmic reticulum protein quality control (ERQC) system has evolved in eukaryotes to ensure accuracy during glycoprotein folding. The human pathogen  Cryptococcus neoformans  possesses a unique  N -glycosylation pathway that affects microbial physiology and interactions with the infected host. To investigate the molecular features and functions of the ERQC system in  C. neoformans,  we characterized a set of mutants with deletion of genes coding for the ERQC sensor UDP-glucose:glycoprotein glucosyltransferase ( UGG1 ) and putative α1,2-mannose-trimming enzymes ( MNS1 ,  MNS101 ,  MNL1 , and  MNL2 ). The  ugg1 Δ,  mns1 Δ,  mns101 Δ, and  mns1 Δ 101 Δ mutants showed alterations in  N -glycan profiles, defective cell surface organization, decreased survival in host cells, and varying degrees of reduced  in vivo  virulence. The  ugg1 Δ strain exhibited severely impaired extracellular secretion of capsular polysaccharides and virulence-related enzymes. Comparative transcriptome analysis showed the upregulation of protein folding, proteolysis, and cell wall remodeling genes, indicative of induced endoplasmic reticulum stress. However, no apparent changes were observed in the expression of genes involved in protein secretion or capsule biosynthesis. Additionally, extracellular vesicle (EV) analysis combined with proteomic analysis showed significant alterations in the number, size distribution, and cargo composition of EVs in  ugg1 Δ. These findings highlight the essential role of the functional ERQC system for cellular fitness under adverse conditions and proper EV-mediated transport of virulence factors, which are crucial for the full fungal pathogenicity of  C. neoformans .

elife

Evolutionary unique  N -glycan-dependent protein quality control system plays pivotal roles in cellular fitness and extracellular vesicle transport in  Cryptococcus neoformans