Open AccessSpectral method of evaluation of photosynthetic apparatus thermal resistance
Author(s) -
V. V. Shevchenko,
Oleksandr Bondarenko
Publication year - 2016
Publication title -
vìsnik ukraïnsʹkogo tovaristva genetikìv ì selekcìonerìv
Language(s) - English
Resource type - Journals
eISSN - 2415-3680
pISSN - 1810-7834
DOI - 10.7124/visnyk.utgis.14.2.694
Subject(s) - photosynthesis , chloroplast , absorption (acoustics) , thermal stability , thermal , chemistry , materials science , botany , horticulture , biology , thermodynamics , physics , biochemistry , composite material , organic chemistry , gene
Aim. The aim of this work is to evaluate the possibilities of application of the spectral parameter - the relationship of the absorption values at wavelengths of 680 and 850 nm (k = A680 / A850) for testing heat resistance of plants to change the size of chloroplasts at short-term heating. Methods. The short-term (5 minutes) heating in the range 25-45oC was carried out for chloroplasts isolated from two-week-old pea, corn and 4 winter wheat varieties differing in heat resistance. From the absorption spectra of chloroplasts was calculated changes of parameter k. Results. All heated chloroplasts showed changes in the spectral parameter k. Particularly strong and stable changes observed during heating at 40-45 ° C. In terms of changes in the spectral parameter plants are ranged in the following order - corn, pea, wheat. Conclusions. The intensity of the k changes coincided with the preliminary estimation of thermal stability for corn and different varieties of winter wheat. It is recommended to use the spectral parameter k for the rapid screening of varieties on the thermal stability of the photosynthetic apparatus within the same species.Key words: chloroplasts of different species of plants, the photosynthetic apparatus, short-term heating, evaluation of the thermal stability.