Open AccessIsolation and Identification of Lactic Acid Bacteria from Traditional Dairy Products in Baotou and Bayannur of Midwestern Inner Mongolia and q-PCR Analysis of Predominant Species
Author(s) -
Dan Wang,
Wenjun Liu,
Yan Ren,
De Liangliang,
Donglei Zhang,
Yanrong Yang,
Qiuhua Bao,
Heping Zhang,
Bilige Menghe
Publication year - 2016
Publication title -
han-gug chugsan sigpum hag-hoeji/korean journal for food science of animal resources
Language(s) - English
Resource type - Journals
eISSN - 2234-246X
pISSN - 1225-8563
DOI - 10.5851/kosfa.2016.36.4.499
Subject(s) - lactococcus , biology , lactococcus lactis , 16s ribosomal rna , food science , leuconostoc mesenteroides , lactic acid , lactobacillus , enterococcus , leuconostoc , multiplex polymerase chain reaction , fermentation , sheep milk , bacteria , subspecies , lactobacillus plantarum , polymerase chain reaction , zoology , biochemistry , genetics , gene
In this study, traditional culture method and 16S rRNA gene analysis were applied to reveal the composition and diversity of lactic acid bacteria (LAB) of fermented cow milk, huruud and urum from Baotou and Bayannur of midwestern Inner Mongolia. Also, the quantitative results of dominant LAB species in three different types of dairy products from Baotou and Bayannur were gained by quantitative polymerase chain reaction (q-PCR) technology. Two hundred and two LAB strains isolated from sixty-six samples were identified and classified into four genera, namely Enterococcus, Lactococcus, Lactobacillus, Leuconostoc, and twenty-one species and subspecies. From these isolates, Lactococcus lactis subsp. lactis (32.18%), Lactobacillus plantarum (12.38%) and Leuconosto mesenteroides (11.39%) were considered as the dominated LAB species under the condition of cultivating in MRS and M17 medium. And the q-PCR results revealed that the number of dominant species varied from samples to samples and from region to region. This study clearly shows the composition and diversity of LAB existing in fermented cow milk, huruud and urum, which could be considered as valuable resources for LAB isolation and further probiotic selection.