MEASUREMENT OF HAEMOCYTES OF GASTROPODS THROUGH AN AUTOMATED IMAGE SCREENING APPROACH BY USING IMAGE PROCESSING TOOL

The present study was screened to determine the number of total haemocytes and measurement (mean radius) of haemocytes, cytoplasm, and nuclei in an image of gastropod haemocytes (Leishman stained) by using Cell Profiler (CP) version 2.1.0; an image analysis tool. The results of primary (nucleus), secondary (haemocyte) and tertiary (cytoplasm) object data, shown that marking of nuclei, haemocytes and cytoplasm by outline and total accepted objects were obtained 23 nos. in the studied image. The area shape of objects especially haemocytes, cytoplasm, and nuclei (arbitrary unit) in an image were also obtained. In conclusion, this study helps to easily screen the total nos. of haemocytes and area (mean radius) measurement of haemocytes, cytoplasm, and nuclei in the image.