Open AccessApplication of gene therapy in the treatment of superficial digital flexor tendon injury in horses
Author(s) -
А. М. Аймалетдинов,
Gulnur Mindubaeva,
Svetlana Khalikova,
Emmanuel Kabwe,
Alexandra Salmakova,
Natalia Alexandrova,
Catrin S. Rutland,
Albert A. Rizvanov,
Elena Zakirova
Publication year - 2020
Publication title -
open veterinary journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.39
H-Index - 10
eISSN - 2226-4485
pISSN - 2218-6050
DOI - 10.4314/ovj.v10i3.3
Subject(s) - tendon , vascular endothelial growth factor , genetic enhancement , medicine , fibroblast growth factor , orthopedic surgery , anatomy , surgery , gene , biology , vegf receptors , genetics , receptor
Background: Tendon injuries are one of the most common causes of orthopedic disorders in horses. Such injuries involve a long course of treatment and recovery. The most promising method of treating these injuries is the use of recombinant proteins and gene therapy.
Aim: In this work, we evaluated the therapeutic efficacy of plasmid DNA (pDNA) containing two species-specific coding sequences, i.e. vascular endothelial growth factor 164 (VEGF164) and fibroblast growth factor 2 (FGF2), in the treatment of severe damage to the tendon of the superficial digital flexor.
Methods: A pDNA construct was used to restore the damaged superficial digital flexor tendon in the horse.
Results: This study showed that the administration of pDNA encoding VEGF164 and FGF2 genes at the injury area increased the regenerative activities of the damaged tendon.
Conclusion: This study shows the therapeutic properties of genetic constructs (pDNA) and contributes to theadvancements in the use of these therapies.
Keywords: Fibroblast growth factor 2, Plasmid DNA, Superficial digital flexor, Vascular endothelial growth factor.