Open AccessFirst molecular detection of <i>Plasmodium relictum</i> in <i>Anopheles sinensis</i> and <i>Armigeres subalbatus</i>
Author(s) -
Jilei Zhang,
Guangyu Lu,
Patrick J. Kelly,
Jing Li,
Min Li,
Jiawei Wang,
Ke Huang,
Haixiang Qiu,
Jinfeng You,
Rong Zhang,
Yaoyao Wang,
Yuanyuan Zhang,
Chengming Wang
Publication year - 2020
Publication title -
open veterinary journal
Language(s) - English
Resource type - Journals
eISSN - 2226-4485
pISSN - 2218-6050
DOI - 10.4314/ovj.v10i1.7
Subject(s) - anopheles sinensis , biology , culex pipiens , avian malaria , culex tritaeniorhynchus , anopheles , plasmodium (life cycle) , culex , vector (molecular biology) , veterinary medicine , malaria , ecology , virology , plasmodium falciparum , gametocyte , parasite hosting , genetics , gene , larva , virus , encephalitis , recombinant dna , world wide web , computer science , immunology , japanese encephalitis , medicine
Background: Plasmodium relictum is one of the most important avian malaria species, which is mainly seen in wild birds, with infections reported in more than 70 different species and at high prevalence.Aim: The aim of this study was to determine the molecular prevalence of Plasmodium spp. in mosquitoes collected in China.Method: A Plasmodium -specific fluorescence resonance energy transfer (FRET) polymerase chain reaction (PCR) was established in this study to analyze five species of mosquitoes (1,620 Culex pipiens pallens, 806 Aedes albopictus, 377 Armigeres subalbatus, 168 Anopheles sinensis, and 80 Culex tritaeniorhynchus) collected in hand nets from homes in 25 provinces of China.Results: Only females originated from six provinces were determined to be positive (0.6%, 10/1,809). Plasmodium species were detected in three mosquito species, such as C. pipiens pallens (0.5%, 8/1,620), A. sinensis (0.6%, 1/168), and A. subalbatus (0.3%, 1/377). Of the three mosquito species positive for P. relictum, only C. pipiens pallens is known to feed on birds and is recognized as the natural vector of P. relictum.Conclusion: This is the first time that P. relictum has been detected in A. sinensis and A. subalbatus. P. relictum, the agent of avian malaria, was present in mosquitoes in China, including mosquito species not previously thought to be the vectors.
Keywords: Anopheles sinensis, Armigeres subalbatus, China, Mosquito, Plasmodium relictum.