Open AccessConstruction of shRNA lentiviral vector
Author(s) -
Hong Sui,
PingChang Yang
Publication year - 2010
Publication title -
north american journal of medical sciences
Language(s) - English
Resource type - Journals
eISSN - 2250-1541
pISSN - 1947-2714
DOI - 10.4297/najms.2010.2598
Subject(s) - small hairpin rna , lentivirus , oligonucleotide , viral vector , insert (composites) , rna interference , vector (molecular biology) , computational biology , virology , biology , medicine , gene , gene knockdown , human immunodeficiency virus (hiv) , genetics , rna , engineering , recombinant dna , viral disease , mechanical engineering
Lentiviruses have been adapted as gene delivery vehicles. This article summarized shRNA lentiviral vector methods generally used in research laboratories. The main procedures of shRNA lentiviral vector include that (1) Target sequences screening and shRNA oligonucleotides designing, (2) insert designed oligonucleotides into lentiviral vectors, (3) using packaging cells to produce shRNA lentivirus, and (4) transducing target cells with shRNA lentivirus.