Open AccessDevelopments in cell culture systems for human pluripotent stem cells
Author(s) -
Weiwei Liu,
Chunhao Deng,
Carlos Godoy-Parejo,
Yumeng Zhang,
Guokai Chen
Publication year - 2019
Publication title -
world journal of stem cells
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.907
H-Index - 18
ISSN - 1948-0210
DOI - 10.4252/wjsc.v11.i11.968
Subject(s) - induced pluripotent stem cell , human induced pluripotent stem cells , stem cell , microbiology and biotechnology , computer science , biology , embryonic stem cell , biochemistry , gene
Human pluripotent stem cells (hPSCs) are important resources for cell-based therapies and pharmaceutical applications. In order to realize the potential of hPSCs, it is critical to develop suitable technologies required for specific applications. Most hPSC technologies depend on cell culture, and are critically influenced by culture medium composition, extracellular matrices, handling methods, and culture platforms. This review summarizes the major technological advances in hPSC culture, and highlights the opportunities and challenges in future therapeutic applications.