Open AccessAnalytical considerations for reducing the matrix effect for the sphingolipidome quantification in whole blood
Author(s) -
Dezhen Wang,
Peining Xu,
Clementina Mesaros
Publication year - 2021
Publication title -
bioanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.566
H-Index - 58
eISSN - 1757-6199
pISSN - 1757-6180
DOI - 10.4155/bio-2021-0098
Subject(s) - sphingolipid , whole blood , lipidomics , metabolomics , analyte , chromatography , ceramide , metabolome , biomarker discovery , chemistry , blood plasma , biomarker , matrix (chemical analysis) , biochemistry , biology , proteomics , immunology , apoptosis , gene
Aim: Plasma and serum are widely used blood-derived biofluids for metabolomics and lipidomics assays, but analytes that are present in high concentrations in blood cells cannot be evaluated in those samples and isolating serum or plasma could introduce additional variability in the data. Materials & methods: In this study, we provide a comprehensive method for quantification of the whole blood (WB) sphingolipidome, combining a single-phase extraction method with LC–high-resolution mass spectrometry. Results: We were able to quantify more than 150 sphingolipids, and when compared with paired plasma, WB contained higher concentration of most sphingolipids and individual variations were lower. These findings suggest that WB could be a better alternative to plasma, and potentially guide the evaluation of the sphingolipidome for biomarker discovery.