Open AccessKerosene: Contributing agent to xylene as a clearing agent in tissue processing
Author(s) -
Amisha Ashokkumar Shah,
Dinraj Kulkarni,
Yashwant Ingale,
Ajit V Koshy,
Sanjay Bhagalia,
Nikhil Bomble
Publication year - 2017
Publication title -
journal of oral and maxillofacial pathology/journal of oral and maxillofacial pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.455
H-Index - 25
eISSN - 1998-393X
pISSN - 0973-029X
DOI - 10.4103/jomfp.jomfp_14_15
Subject(s) - clearing , kerosene , xylene , business , waste management , computer science , chemistry , engineering , organic chemistry , finance , toluene
Research methodology in oral and maxillofacial pathology has illimitable potential. The tissue processing involves many steps of which one of the most important step is "Clearing," which is a process of replacing dehydrant with a substance which is miscible with embedding medium or paraffin wax. Xylene is one of the common clearing agents used in laboratory, but it is also hazardous. The main aim of this study is to substitute conventionally used xylene by a mixture of kerosene and xylene in clearing steps without altering the morphology and staining characteristics of tissue sections. This will also minimize the toxic effects and tend to be more economical.