Open AccessThe purity identification and radiolabeling of α-mangostin with technetium-99m
Author(s) -
Muchtaridi Muchtaridi,
Luthfi Utami Setyawati,
Risda Rahmi Islamiaty,
Kevin Reinard Lie,
Wiwit Nurhidayah
Publication year - 2020
Publication title -
journal of advanced pharmaceutical technology and research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.325
H-Index - 33
eISSN - 2231-4040
pISSN - 0976-2094
DOI - 10.4103/japtr.japtr_94_19
Subject(s) - high performance liquid chromatography , chromatography , chemistry , technetium , thin layer chromatography , column chromatography , breast cancer , radiochemistry , cancer , nuclear chemistry , medicine
Alpha-mangostin (AM) is a natural compound that has the greatest activity in breast cancer. Radiolabeling AM with technetium-99 m (Tc-99m) has a function as breast cancer radiotracer. This study is aimed to identify the purity of Tc-99m-labeled AM. The identification method was conducted by a validated radio-high-performance liquid chromatography (HPLC) to confirm the chemical purity of the compound when the thin layer of chromatography and paper chromatography were used to find out the radiochemical purity (RCP). The validated radio-HPLC method obtained was C18 column with methanol:water (90:10) as the mobile phase and ultraviolet (243 nm) tandem radioactive detector (Gabi Star). The result showed that the RCP was 70.6% ± 2.87%. The analytical method met the validation criteria according to ICH Q2 (R1); thus, it could be applied in the identification. Unfortunately, the 99m Tc-AM identification using radio-HPLC showed that the expected complex was not yet formed perfectly because of chemical impurities.