Open AccessThe cytotoxicity test of calcium hydroxide, propolis, and calcium hydroxide-propolis combination in human pulp fibroblast
Author(s) -
Ira Widjiastuti,
Mieke Kusuma Dewi,
Edhi Arief Prasetyo,
Nirawati Pribadi,
Mochamad Moedjiono
Publication year - 2020
Publication title -
journal of advanced pharmaceutical technology and research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.325
H-Index - 33
eISSN - 2231-4040
pISSN - 0976-2094
DOI - 10.4103/japtr.japtr_88_19
Subject(s) - propolis , calcium hydroxide , pulp capping , pulp (tooth) , chemistry , fibroblast , calcium , food science , nuclear chemistry , biochemistry , dentistry , in vitro , medicine , organic chemistry
Calcium hydroxide (Ca(OH) 2 ) is the gold standard material used for pulp-capping but still has a high failure rate. Thus, an alternative material is needed, one of which is propolis. The combination of Ca(OH) 2 propolis is expected to have better quality and to be biocompatible. The aim of this study is to investigate the viability of human pulp fibroblast after the administration of Ca(OH) 2 , propolis, and its combination. Human pulp fibroblast culture derived from premolar teeth of 16-year-old patients, were divided into seven groups: Group 1 (10 μg Ca(OH) 2 ); Group 2 (10 μg propolis); Group 3 (15 μg propolis); Group 4 (20 μg propolis); Group 5 (Ca(OH) 2 -propolis 1:1); Group 6 (Ca(OH) 2 -propolis 1:1.5); and Group 7 (calcium hydroxide-propolis 1:2). They were placed in a 96 wells plate and put into incubator for 24 h. The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide test was conducted to calculate the viability of human pulp fibroblasts. The data were analyzed statistically using Kolmogorov-Smirnov, Levene's test, one-way analysis of variance, and Tukey-honestly significant difference ( P < 0.05). The number of living human pulp fibroblast after the administration of Ca(OH) 2 and propolis combination is greater than the application of Ca(OH) 2 or propolis with significant different between groups ( P < 0.05). The viability of human pulp fibroblasts after the administration of Ca(OH) 2 -propolis combination is greater than that of the application of Ca(OH) 2 and propolis alone.