Open AccessCloning, expression & evaluation of potential immunogenic recombinant capsid premembrane protein of West Nile virus
Author(s) -
Jyoti Kumar,
Sivakumar R. Rathinam,
Divanyshi Karothia,
Manmohan Parida
Publication year - 2019
Publication title -
indian journal of medical research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.578
H-Index - 87
ISSN - 0971-5916
DOI - 10.4103/ijmr.ijmr_305_17
Subject(s) - virology , flavivirus , dengue fever , capsid , recombinant dna , medicine , immunoglobulin m , virus , encephalitis , flaviviridae , antibody , biology , immunoglobulin g , immunology , viral disease , gene , biochemistry
West Nile virus (WNV) is a neurotropic flavivirus that has emerged globally as a significant cause of viral encephalitis. The early confirmatory diagnosis of WNV infections is important for timely clinical management and in areas where multiple flaviviruses are endemic. Diagnosis of WNV infection is primarily based on serodiagnosis, followed by virus isolation and identification. The aim of this study was to develop and evaluate a highly sensitive and specific immunoglobulin M (IgM) ELISA using the recombinant CprM protein (rWNV-CprM) for rapid, early and accurate diagnosis of WNV.