Open AccessDevelopment of indigenous IgG ELISA for the detection of anti-SARS-CoV-2 IgG
Author(s) -
PragyaD Yadav,
Gajanan Sapkal,
Anita Shete-Aich,
Rajlaxmi Jain,
Prasad Sarkale,
Rajen Lakra,
Srikant Baradkar,
GururajRao Deshpande,
Deepak Mali,
BipinN Tilekar,
Triparna Majumdar,
Himanshu Kaushal,
Yogesh K. Gurav,
Nivedita Gupta,
Sreelekshmy Mohandas,
Ketki Deshpande,
Ojas Kaduskar,
Malvika Salve,
Savita Patil,
Shivshankar Gaikwad,
A P Sugunan,
M Ashok,
Sidhartha Giri,
Jayanthi Shastri,
Priya Abraham,
RamanR Gangakhedkar,
COVID Support Team
Publication year - 2020
Publication title -
indian journal of medical research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.578
H-Index - 87
ISSN - 0971-5916
DOI - 10.4103/ijmr.ijmr_2232_20
Subject(s) - coronaviridae , virology , coronavirus , outbreak , medicine , betacoronavirus , serology , covid-19 , virus , pandemic , antibody , immunology , disease , infectious disease (medical specialty)
Since the beginning of the year 2020, the pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) impacted humankind adversely in almost all spheres of life. The virus belongs to the genus Betacoronavirus of the family Coronaviridae. SARS-CoV-2 causes the disease known as coronavirus disease 2019 (COVID-19) with mild-to-severe respiratory illness. The currently available diagnostic tools for the diagnosis of COVID-19 are mainly based on molecular assays. Real-time reverse transcription-polymerase chain reaction is the only diagnostic method currently recommended by the World Health Organization for COVID-19. With the rapid spread of SARS-CoV-2, it is necessary to utilize other tests, which would determine the burden of the disease as well as the spread of the outbreak. Considering the need for the development of such a screening test, an attempt was made to develop and evaluate an IgG-based ELISA for COVID-19.