Open AccessDNA extraction from nocardia species for special genes analysis using PCR
Author(s) -
Mehdi Fatahi Bafghi,
Seyyed Saeed Eshraghi,
Parvin Heidarieh,
Shadi Habibnia,
Masoumeh Rasouli Nasab
Publication year - 2014
Publication title -
north american journal of medical sciences
Language(s) - English
Resource type - Journals
eISSN - 2250-1541
pISSN - 1947-2714
DOI - 10.4103/1947-2714.132943
Subject(s) - polymerase chain reaction , dna extraction , 16s ribosomal rna , genomic dna , nocardia , dna , agarose , lysis , biology , microbiology and biotechnology , gene , alkaline lysis , polymerase , chromatography , bacteria , chemistry , biochemistry , genetics , plasmid , dna vaccination
Nocardia species have a complex cell wall structure similar to that of mycobacteria, and the extraction of DNA from this bacterium is extremely difficult. Currently, to identify Nocardia species particularly, it is essential to utilize molecular techniques.