Open AccessDynamic changes in the systemic immune responses of spinal cord injury model mice
Author(s) -
Tingting Gao,
Feifei Huang,
Yuanyuan Xie,
Wenqing Wang,
Liudi Wang,
Dan Mu,
Yi Cui,
Bin Wang
Publication year - 2021
Publication title -
neural regeneration research/neural regeneration research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.93
H-Index - 38
eISSN - 1876-7958
pISSN - 1673-5374
DOI - 10.4103/1673-5374.290910
Subject(s) - spinal cord injury , immune system , spinal cord , medicine , neuroscience , immunology , biology
Intraspinal inflammatory and immune responses are considered to play central roles in the pathological development of spinal cord injury. This study aimed to decipher the dynamics of systemic immune responses, initiated by spinal cord injury. The spinal cord in mice was completely transected at T8. Changes in the in vivo inflammatory response, between the acute and subacute stages, were observed. A rapid decrease in C-reactive protein levels, circulating leukocytes and lymphocytes, spleen-derived CD4 + interferon-γ + T-helper cells, and inflammatory cytokines, and a marked increase in neutrophils, monocytes, and CD4 + CD25 + FOXP3 + regulatory T-cells were observed during the acute phase. These systemic immune alterations were gradually restored to basal levels during the sub-acute phase. During the acute phase of spinal cord injury, systemic immune cells and factors showed significant inhibition; however, this inhibition was transient, and the indicators of these serious disorders gradually returned to baseline levels during the subacute phase. All experiments were performed in accordance with the institutional animal care guidelines, approved by the Institutional Animal Care and Use Committee of Experimental Animal Center of Drum Tower Hospital, China (approval No. 2019AE01040) on June 25, 2019.