Open AccessHuman umbilical cord mesenchymal stem cells promote peripheral nerve repair via paracrine mechanisms
Author(s) -
Zhixin Guo,
Xiaojuan Sun,
Xiaolong Xu,
Qing Zhao,
Jiang Peng,
Yu Wang
Publication year - 2015
Publication title -
neural regeneration research/neural regeneration research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.93
H-Index - 38
eISSN - 1876-7958
pISSN - 1673-5374
DOI - 10.4103/1673-5374.155442
Subject(s) - paracrine signalling , neurotrophic factors , mesenchymal stem cell , microbiology and biotechnology , nerve growth factor , basic fibroblast growth factor , hepatocyte growth factor , glial cell line derived neurotrophic factor , regeneration (biology) , stem cell , nerve guidance conduit , neurotrophin , growth factor , biology , medicine , receptor
Human umbilical cord-derived mesenchymal stem cells (hUCMSCs) represent a promising young-state stem cell source for cell-based therapy. hUCMSC transplantation into the transected sciatic nerve promotes axonal regeneration and functional recovery. To further clarify the paracrine effects of hUCMSCs on nerve regeneration, we performed human cytokine antibody array analysis, which revealed that hUCMSCs express 14 important neurotrophic factors. Enzyme-linked immunosorbent assay and immunohistochemistry showed that brain-derived neurotrophic factor, glial-derived neurotrophic factor, hepatocyte growth factor, neurotrophin-3, basic fibroblast growth factor, type I collagen, fibronectin and laminin were highly expressed. Treatment with hUCMSC-conditioned medium enhanced Schwann cell viability and proliferation, increased nerve growth factor and brain-derived neurotrophic factor expression in Schwann cells, and enhanced neurite growth from dorsal root ganglion explants. These findings suggest that paracrine action may be a key mechanism underlying the effects of hUCMSCs in peripheral nerve repair.