Open AccessLiquid chromatographic determinations of meta-chlorobenzoic acid in bupropion hydrochloride
Author(s) -
Sukumar Sinha,
Vikas Parashar,
K Swamy Reddy,
M. A. Khan,
Sk Manirul Haque
Publication year - 2013
Publication title -
journal of natural science, biology and medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.236
H-Index - 30
eISSN - 2229-7707
pISSN - 0976-9668
DOI - 10.4103/0976-9668.117018
Subject(s) - chemistry , chromatography , absorbance , hydrochloride , ethanol , correlation coefficient , hexane , linear correlation , linear relationship , organic chemistry , mathematics , statistics
A simple, sensitive method for the determination of meta-chlorobenzoic acid in bupropion hydrochloride is described. Chromatographic separation of m-chlorobenzoic acid is achieved using a mobile phase consisting of n-hexane and ethanol (1000:50, v/v) at a flow rate of 1.0 ml/min on a Chiralpak ADH (250 × 4.6 mm). Absorbance is monitored at 235 nm. The method is linear for m-chlorobenzoic acid over concentration range of LOQ, 5.0 μg/ml to 15.0 μg/ml for m-chlorobenzoic acid with correlation coefficient greater than 0.99. This method is more selective and accurate than United States Pharmacopoeia method for the determination of m-chlorobenzoic acid in bupropion hydrochloride.