Open AccessCloning, sequencing, and in silico characterization of Omp 28 of Salmonella Typhi (strain MTCC 733) to develop r-DNA vaccine for typhoid fever
Author(s) -
Amita Saxena,
Shantanu Tamuly,
Mumtesh Kumar Saxena
Publication year - 2012
Publication title -
journal of natural science, biology and medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.236
H-Index - 30
eISSN - 2229-7707
pISSN - 0976-9668
DOI - 10.4103/0976-9668.101885
Subject(s) - salmonella typhi , dna vaccination , biology , reverse vaccinology , epitope , in silico , virology , antigen , amplicon , molecular cloning , typhoid fever , recombinant dna , gene , microbiology and biotechnology , genetics , peptide sequence , polymerase chain reaction , escherichia coli
Typhoid is one of the most important diseases of human beings caused by Salmonella Typhi. There are many vaccine reported against Salmonella Typhi, but search for new candidate vaccine antigens is still going on because presently available vaccines have several limitations such as short-term immunity, high cost, and allergic reaction. Several approaches such as subunit vaccines, Vi polysaccharide, mutant vaccines, and r-DNA vaccines have been tested. r- DNA vaccines have shown some promising potential (targeted Omp). Omp 28 had shown very promising results and suggests that it should be used in further studies of animal protection against the disease.