Open AccessDetermination of sex by exfoliative cytology using acridine orange confocal microscopy: A short study
Author(s) -
Dorankula Shyam Prasad Reddy,
Herald J Sherlin,
Pratibha Ramani,
P Prakash
Publication year - 2012
Publication title -
journal of forensic dental sciences
Language(s) - English
Resource type - Journals
eISSN - 0975-2137
pISSN - 0975-1475
DOI - 10.4103/0975-1475.109887
Subject(s) - acridine orange , exfoliative cytology , confocal , buccal mucosa , staining , confocal microscopy , microscope slide , pathology , cytology , confocal laser scanning microscopy , buccal swab , biology , microscope , medicine , dentistry , microbiology and biotechnology , optics , biophysics , physics , oral cavity
Establishing individuality is an imperative aspect in any investigation procedure. Sometimes, in identifying an individual, it becomes necessary to determine the sex of that particular individual. Combining rapidity with reliability, an innovative idea has been put forward using a confocal microscope in exfoliative cytology. In the present study, we have determined the sex of the individual from buccal mucosal scrapings. The exfoliative cells were observed for Barr bodies under a confocal microscope, and the percentage of Barr-body-positive cells was determined.